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  • 細胞凋亡的生物學意義主要表現(xiàn)在:①細胞凋亡在生物界中普遍存在。②細胞凋亡是多細胞生物體的生命活動中不-可-缺-少的組成部分,是其自身借以存活的需要,因而貫穿于全部生命周期中,無論是低等動物還是高等動物均是如此。③細胞凋亡是生物在進化過程中所...

  • 內(nèi)毒素進入機體后,99%很快被清除。內(nèi)毒素在體內(nèi)、體外均可引發(fā)免疫反應(yīng),使單核-巨噬細胞等細胞釋出細胞因子,發(fā)揮內(nèi)毒素的生物學作用,其中涉及誘導細胞死亡,包括細胞凋亡和細胞壞死。機體內(nèi)細胞死亡通??煞譃閮深悾阂活愂羌毎膲乃溃锢硇?、化學性...

  • 天然BPI對各種光滑型革蘭陰性菌外膜LPS的親和力有很大差異,而對各種純化的游離LPS的親和力則基本相同。這表明細菌外膜上LPS分子的表型與游離LPS分子存在差異。前者通過脂質(zhì)A區(qū)結(jié)合的長鏈脂肪酸﹐借疏水鍵與細菌外膜脂質(zhì)雙分子層的外側(cè)緊密相...

  • 凝膠法鱟試劑是生物化學和分子生物學領(lǐng)域常用的實驗試劑,廣泛應(yīng)用于核酸電泳、蛋白質(zhì)分離和檢測等方面。下面將介紹鱟試劑的反應(yīng)原理以及在科研和臨床應(yīng)用中的重要性。這是一種緩沖溶液,其中包含聚合物和離子,可以形成凝膠基質(zhì),并提供適當?shù)膒H和離子強度...

  • 殺菌滲透增強蛋白對革蘭陰性菌的毒性作用表現(xiàn)為兩個階段,即早期可逆性損傷階段和晚期不可逆性損傷階段。(1)殺菌滲透增強蛋白通過其口袋與革蘭陰性菌膜上的LPS等磷脂成分結(jié)合,造成直接的細菌生長停滯;生長停滯與細胞外膜的改變有關(guān),兩者的效應(yīng)起初為...

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  • 大多數(shù)學者認為,內(nèi)化反應(yīng)和激活效應(yīng)是分離的。最近Beutler等證實,具有特異性的抗TLR4多克隆抗體具有擬內(nèi)毒素的活性,從而認為LPS并非必須經(jīng)過內(nèi)化才可誘發(fā)信號轉(zhuǎn)導效應(yīng)。Kitchens等證實,mCD14介導的LPS內(nèi)化的動力學主要受L...

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